Adipose tissue was first used during the First World War to promote the healing of soldiers’ wounds.
After a century, researchers have discovered that fat is one of the richest adult tissues in mesenchymal stem cells frequency.
These cells can differentiate into specialized cells, but even more important, they can respond to local stimuli coming from a degenerated tissue and release molecules such as growth factors and anti-inflammatory cytokines to promote healing.
Lipo-Vet is a technology able to enhance the biological properties of adipose tissue.
TECHNOLOGY
Lipo-Med is equipped with a semipermeable membrane that separates adipose tissue from waste elements with the help of a continuous irrigation.
The dialysis of the tissue minimizes the stress and trauma to cell and extracellular matrix architecture, removing the blood and oil residues which are pro-inflammatory.
The final product is a purified adipose tissue reduced into clusters.
FEATURES
- MINIMAL MECHANICAL STRESS
to maintain the biochemical properties of cells and the integrity of extracellular matrix. - TOTAL PURIFICATION
from blood and oil residues that can possibly lead to inflammation. - MINIMAL MANIPULATION
of the tissue granted by a “point-of-care” technology that performs intraoperatively - CLOSED-LOOP CIRCUIT
and a procedure completely performed in the sterile field
Histology of untreated adipose tissue (NT), Lipo-VET processed (LC), and centrifuged (CF) lipoaspirate centrifugation. The hematoxylin eosin staining shows the maintenance of tissue architecture integrity, see LC example.
INFILTRATION
The infiltration aims to prepare for the adipose tissue liposuction.
The saline, while promoting more vasoconstriction through pressure increase, creates a tumescent area that help the liposuction with the provided aspirating cannulae.
After performing an incision in the illustrated spots, use the infiltration cannula (16G) connected to 60 ml syringes pre-filled with Klein solution. It is very important to perform the infiltration homogeneously during retrograde movements of the cannulla. Avoid transverse movements with the cannulas. After the infiltration of 150-200 ml, wait 10 minutes. It is possible to perform a digital manipolation of the abdomen to help the distribution of the solution.
KLEIN SOLUTION
A mixture of sterile saline 4ml/kg b.wt. and adrenalin 0.05 mg/10 ml NaCI solution
ASPIRATION
After 10 minutes, it is possible to connect the aspirating cannula (13G) to the self-blocking syringe.
The lock system, that must be activated while the cannula isinside the subcutaneous adipose panniculus, creates negative pressure inside the syringe.
Moving the syring back and forth the lipoaspirate is harvested from the previously infiltrated areas.
Avoid transverse movements with the cannulas.
- Connect the Lipo-VET device to a washing
bag (Ringer Lactate is recommended) with a
minimum volume of 500 ml, hanging from a stand. Drop the waste bag instead Insert the obtained lipoaspirate through the “IN”
luer-lock connection. - Open the irrigation valve and facilitate the washing of the tissue. By gently moving
and pressing on the bag with the provided spatula. Keep on moving until the tissue becomes tipically yellow and the outflow
irrigation is almost trasparent - Close the irrigation valve and remove the excess washing solution with the help of the brush. Connect a 10 ml syringe to the “OUT” luer- lock connection and collect the final product ready-to-use.
It is recommended not to manipulate the tissue and to use it as needed. If the product obtained appears very thick, transfer it into the 2.5 ml syringes supplied with the kit or into smaller luer-lock syringes to facilitate the release of the product during grafting. It is pollible to use the needle in the kit or other needles with a recommended diameter of 18 or 20G.
HIGH REGENERATIVE POTENTIAL
The atraumatic processing of the tissue limits the cell stress, thus not impairing their trofic and anti- inflammatory activity.
An undisrupted extracellular matrix is able to act as a natural scaffold for cells by improving their vitality and contributing to tissue regeneration.
The removal of blood and oil from adipose tissue limits the stress and inflammation of the tissue hosting the graft.
A SIMPLE, EFFECTIVE, AND SAFE PROCEDURE
The system is a closed-loop circuit and the procedure is completely performed in the sterile field, minimizing the risk of contaminations.
The device fulfills the requirements of cell and tissue minimal manipulation.
The procedure is simple, fast, and reproducible, being versatile in different therapeutic fields.
HUMAN RESULT
Lipo-Med processing combined with Ringer Lactate washing completely preserves the stemness potential of adipose tissue’s mesenchymal cells.
ASCs were isolated and counted immediately after Lipo-Med processing (1) or after cell culture (2 passages) (2)
Data show fold increase of cell yield (1) and stemness preservation percentage (2) over control (untreated lipoaspirate).
